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BIA Separations is now part of Sartorius Stedim Biotech. Learn more >
Ways to improve empty, full and damaged capsids separation
 
Last month we attended the 'Gene Therapy for Rare Disorders Europe 2020' conference, which ran virtually on October 26 and 27. On the second day of the meeting, our CEO Ales Strancar presented methods for improving separation of AAV empty, full, and other AAV related particles /capsids. In his talk, he highlighted that buffer optimization and proper column ligand selection (CIM QA, CIM PrimaS, or new CIM EF) allow for >90% full capsid production. He introduced Specimen and emphasized the ultracentrifuge usage with HPLC detectors for prompt orthogonal verification of the maximum capsid purity. You can download his presentation here.
BIA Separations won the Creating for CenturiesAward!
We are proud to announce that we received 'The Creating for Centuries Award' for the development of ideas, innovations, and investments in Entrepreneurship in Central and SE Europe for 2019. International Economy Forum 'Perspective' recognized our value and awarded us for our contribution to the creativity, innovation and education of entrepreneurs as well as innovation and investment in promising projects. We toast to this success and look forward to our future accomplishments. 
Exploring CIMac PrimaS™ for Analysis of mRNA

CIMac PrimaS is a new member of BIA’s family of high-performance chromatography products to analyse and purify mRNA. While QA and DEAE anion exchangers need to be heated in the range of 50–70°C for large mRNA to elute, new experimental data show that one can also elute large mRNA from CIMac PrimaS at ambient temperature in a pyrophosphate gradient.
New poster - Fast HPLC analytics to characterise IVT reaction - detection of pDNA, mRNA, and IVT components
At the beginning of the month, we attended the 8th International mRNA Health Conference, where we presented two new posters. The first one introduces a new analytical HPLC approach that uses CIMac PrimaS for separation of IVT components such as triphosphate-nucleotides (NTPs), enzymes, DNA template, and RNA in a very short gradient.
New poster - Preparation of linear plasmid DNA for in vitro transcription reaction
 
The second poster describes a single-step-capture strategy to maximize the recovery of pDNA for further linearization. The image on left shows comparison of the HPLC profile of pDNA before (dashed line) and after linearization (solid line).
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